NeoTherm™ DNA POLYMERASE is a thermostable DNA polymerase purified from the Thermus aquaticus strain in accordance with the procedures developed by Kaledin for the isolation from thermophilic bacteria of thermostable enzymesAPPLICATIONCONCENTRATION
processing DNA polymerase activity.
It is a highly processive 5'-3' DNA polymerase lacking 3'-5'-exonuclease activity. The enzyme is highly puri-fied
and is free of nonspecific endo- or exonucleases.
This polymerase has a template-dependent activity which adds a single deoxyadenosine (A) to the 3' ends
of PCR products (3'-A-overhangs). This property allows easy and efficient ligation of PCR products in TA clo-ning
5 units/µlUNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 min at 72°C.STORAGE BUFFER
20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol, 1% Triton X-100STORAGE TEMPERATURE
-20°C10X REACTION BUFER
160 mM (NH4)2SO4, 670 mM Tris-HCl pH 8.8 (at 25ºC), 15 mM MgCl2, 0.1% Tween 20QUALITY CONTROL
The 10x reaction buffer (on request with or without MgCl2) is delivered free of charge.
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases
The enzyme is also tested to verify that less than 10 copies of bacterial 16S ribosomal RNA gene sequences
are present in a standard 2.5 unit aliquot.